rabbit anti human p53 antibody Search Results


human/mouse/rat p53 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation human/mouse/rat p53 antibody
Human/Mouse/Rat P53 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human/mouse/rat p53 antibody/product/Bio-Techne corporation
Average 99 stars, based on 1 article reviews
human/mouse/rat p53 antibody - by Bioz Stars, 2026-03
99/100 stars
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rabbit anti-human p53 monoclonal antibody  (Becton Dickinson)
90
Becton Dickinson rabbit anti-human p53 monoclonal antibody
H&E staining of <t>p53</t> <t>protein</t> in the three experimental groups (from left to right: para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue). Samples obtained from para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue were stained using H&E staining method. There was p53 protein expression in para-cancerous tissue, increased expression in non-metastatic lung cancer tissues and abundant expression in metastatic spinal tumor tissue.
Rabbit Anti Human P53 Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human p53 monoclonal antibody/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
rabbit anti-human p53 monoclonal antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
antibody against acetyl-p53 (lys320, 06–1283)  (Merck KGaA)
90
Merck KGaA antibody against acetyl-p53 (lys320, 06–1283)
Cisplatin induces K472 acetylation and S461 phosphorylation of PFKFB3. a DNA damage signals induced PFKFB3 K472 acetylation. Flag-tagged PFKFB3 was expressed in HEK293T cells, which were then treated with etoposide (10 μM), adriamycin (1 μM), UV irradiation (10 J/m 2 ) and cisplatin (50 or 100 μM) for 24 h. Flag-PFKFB3 was immunoprecipitated with Flag beads and immunoblotting was performed with the antibodies indicated. Relative PFKFB3 K472 acetylation and phosphorylation were normalized by Flag protein. b The amino acid sequence near K472 of PFKFB3 displays high similarity with the sequence near K320 of <t>TP53.</t> c Cisplatin treatment induces PFKFB3 cytoplasmic accumulation. HEK293T cells were treated with or without cisplatin (50 μM) for 24 h before harvest. Cells were then suspended in PBS and treated with a gradient concentration of digitonin. Supernatant and precipitate were collected for immunoblotting with indicated antibodies. S, supernatant; P, precipitate. d Cisplatin induces K472 acetylation and S461 phosphorylation of endogenous PFKFB3. Endogenous PFKFB3 protein was purified from HEK293T cells after cisplatin treatment as indicated for 24 h. e Cisplatin or etoposide treatment enhances K472 acetylation in SIRT1 knockout cells. Endogenous PFKFB3 protein were purified from WT or SIRT1 knockout HEK293T cells treated with EX527 (10 μM), cisplatin (50 μM) or etoposide (10 μM) for 24 h. f Combined knockdown of PCAF and GCN5 abolishes cisplatin- or etoposide-induced PFKFB3 K472 acetylation. HEK293T cells were transfected with siRNAs targeting PCAF and GCN5. After 60 h, cells were treated with cisplatin (50 μM) or etoposide (10 μM) for 24 h. g , h Cisplatin treatment induces pan-acetylation of PCAF and GCN5. Flag-tagged PCAF or GCN5 was expressed in HEK293T cells, which were treated with cisplatin for the duration indicated at a concentration of 50 μM. Relative pan-acetylation level of PCAF or GCN5 was normalized by Flag protein. i Cisplatin increases acetyltransferase activity of PCAF and GCN5. Flag-tag PCAF or GCN5 was purified from HEK293T cells treated with or without cisplatin (50 μM) for 24 h, then incubated with recombinant His-PFKFB3 in acetylation assay buffer. Purified proteins visualized by Coomassie blue staining are shown (lower panel). Data are representative of at least two independent experiments
Antibody Against Acetyl P53 (Lys320, 06–1283), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody against acetyl-p53 (lys320, 06–1283)/product/Merck KGaA
Average 90 stars, based on 1 article reviews
antibody against acetyl-p53 (lys320, 06–1283) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-p53 rabbit anti-human/mouse antibody e11-10276c  (EnoGene Inc)
90
EnoGene Inc anti-p53 rabbit anti-human/mouse antibody e11-10276c
Cisplatin induces K472 acetylation and S461 phosphorylation of PFKFB3. a DNA damage signals induced PFKFB3 K472 acetylation. Flag-tagged PFKFB3 was expressed in HEK293T cells, which were then treated with etoposide (10 μM), adriamycin (1 μM), UV irradiation (10 J/m 2 ) and cisplatin (50 or 100 μM) for 24 h. Flag-PFKFB3 was immunoprecipitated with Flag beads and immunoblotting was performed with the antibodies indicated. Relative PFKFB3 K472 acetylation and phosphorylation were normalized by Flag protein. b The amino acid sequence near K472 of PFKFB3 displays high similarity with the sequence near K320 of <t>TP53.</t> c Cisplatin treatment induces PFKFB3 cytoplasmic accumulation. HEK293T cells were treated with or without cisplatin (50 μM) for 24 h before harvest. Cells were then suspended in PBS and treated with a gradient concentration of digitonin. Supernatant and precipitate were collected for immunoblotting with indicated antibodies. S, supernatant; P, precipitate. d Cisplatin induces K472 acetylation and S461 phosphorylation of endogenous PFKFB3. Endogenous PFKFB3 protein was purified from HEK293T cells after cisplatin treatment as indicated for 24 h. e Cisplatin or etoposide treatment enhances K472 acetylation in SIRT1 knockout cells. Endogenous PFKFB3 protein were purified from WT or SIRT1 knockout HEK293T cells treated with EX527 (10 μM), cisplatin (50 μM) or etoposide (10 μM) for 24 h. f Combined knockdown of PCAF and GCN5 abolishes cisplatin- or etoposide-induced PFKFB3 K472 acetylation. HEK293T cells were transfected with siRNAs targeting PCAF and GCN5. After 60 h, cells were treated with cisplatin (50 μM) or etoposide (10 μM) for 24 h. g , h Cisplatin treatment induces pan-acetylation of PCAF and GCN5. Flag-tagged PCAF or GCN5 was expressed in HEK293T cells, which were treated with cisplatin for the duration indicated at a concentration of 50 μM. Relative pan-acetylation level of PCAF or GCN5 was normalized by Flag protein. i Cisplatin increases acetyltransferase activity of PCAF and GCN5. Flag-tag PCAF or GCN5 was purified from HEK293T cells treated with or without cisplatin (50 μM) for 24 h, then incubated with recombinant His-PFKFB3 in acetylation assay buffer. Purified proteins visualized by Coomassie blue staining are shown (lower panel). Data are representative of at least two independent experiments
Anti P53 Rabbit Anti Human/Mouse Antibody E11 10276c, supplied by EnoGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-p53 rabbit anti-human/mouse antibody e11-10276c/product/EnoGene Inc
Average 90 stars, based on 1 article reviews
anti-p53 rabbit anti-human/mouse antibody e11-10276c - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-human p53 monoclonal antibody do7  (Novocastra)
90
Novocastra rabbit anti-human p53 monoclonal antibody do7
Cisplatin induces K472 acetylation and S461 phosphorylation of PFKFB3. a DNA damage signals induced PFKFB3 K472 acetylation. Flag-tagged PFKFB3 was expressed in HEK293T cells, which were then treated with etoposide (10 μM), adriamycin (1 μM), UV irradiation (10 J/m 2 ) and cisplatin (50 or 100 μM) for 24 h. Flag-PFKFB3 was immunoprecipitated with Flag beads and immunoblotting was performed with the antibodies indicated. Relative PFKFB3 K472 acetylation and phosphorylation were normalized by Flag protein. b The amino acid sequence near K472 of PFKFB3 displays high similarity with the sequence near K320 of <t>TP53.</t> c Cisplatin treatment induces PFKFB3 cytoplasmic accumulation. HEK293T cells were treated with or without cisplatin (50 μM) for 24 h before harvest. Cells were then suspended in PBS and treated with a gradient concentration of digitonin. Supernatant and precipitate were collected for immunoblotting with indicated antibodies. S, supernatant; P, precipitate. d Cisplatin induces K472 acetylation and S461 phosphorylation of endogenous PFKFB3. Endogenous PFKFB3 protein was purified from HEK293T cells after cisplatin treatment as indicated for 24 h. e Cisplatin or etoposide treatment enhances K472 acetylation in SIRT1 knockout cells. Endogenous PFKFB3 protein were purified from WT or SIRT1 knockout HEK293T cells treated with EX527 (10 μM), cisplatin (50 μM) or etoposide (10 μM) for 24 h. f Combined knockdown of PCAF and GCN5 abolishes cisplatin- or etoposide-induced PFKFB3 K472 acetylation. HEK293T cells were transfected with siRNAs targeting PCAF and GCN5. After 60 h, cells were treated with cisplatin (50 μM) or etoposide (10 μM) for 24 h. g , h Cisplatin treatment induces pan-acetylation of PCAF and GCN5. Flag-tagged PCAF or GCN5 was expressed in HEK293T cells, which were treated with cisplatin for the duration indicated at a concentration of 50 μM. Relative pan-acetylation level of PCAF or GCN5 was normalized by Flag protein. i Cisplatin increases acetyltransferase activity of PCAF and GCN5. Flag-tag PCAF or GCN5 was purified from HEK293T cells treated with or without cisplatin (50 μM) for 24 h, then incubated with recombinant His-PFKFB3 in acetylation assay buffer. Purified proteins visualized by Coomassie blue staining are shown (lower panel). Data are representative of at least two independent experiments
Rabbit Anti Human P53 Monoclonal Antibody Do7, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human p53 monoclonal antibody do7/product/Novocastra
Average 90 stars, based on 1 article reviews
rabbit anti-human p53 monoclonal antibody do7 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


H&E staining of p53 protein in the three experimental groups (from left to right: para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue). Samples obtained from para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue were stained using H&E staining method. There was p53 protein expression in para-cancerous tissue, increased expression in non-metastatic lung cancer tissues and abundant expression in metastatic spinal tumor tissue.

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: H&E staining of p53 protein in the three experimental groups (from left to right: para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue). Samples obtained from para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue were stained using H&E staining method. There was p53 protein expression in para-cancerous tissue, increased expression in non-metastatic lung cancer tissues and abundant expression in metastatic spinal tumor tissue.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Staining, Expressing

p53 protein expression in three tissue sample groups.

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: p53 protein expression in three tissue sample groups.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing

Correlation of MMP-9 and p53 protein expression in lung cancer metastatic spinal tumor tissues.

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: Correlation of MMP-9 and p53 protein expression in lung cancer metastatic spinal tumor tissues.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing, Significance Assay

Patient survival curves for MMP-9 and p53 protein expression positive and negative groups. (A) The survival rate of patients with negative p53 protein expression was significantly higher than that of patients with a positive p53 expression (χ 2 =9.826, p<0.01). (B) The survival rate of patients with a negative MMP-9 protein expression was higher than that of MMP-9 protein-positive patients (χ 2 =4.436, p<0.05). MMP-9, matrix metalloproteinase-9.

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: Patient survival curves for MMP-9 and p53 protein expression positive and negative groups. (A) The survival rate of patients with negative p53 protein expression was significantly higher than that of patients with a positive p53 expression (χ 2 =9.826, p<0.01). (B) The survival rate of patients with a negative MMP-9 protein expression was higher than that of MMP-9 protein-positive patients (χ 2 =4.436, p<0.05). MMP-9, matrix metalloproteinase-9.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing

Cisplatin induces K472 acetylation and S461 phosphorylation of PFKFB3. a DNA damage signals induced PFKFB3 K472 acetylation. Flag-tagged PFKFB3 was expressed in HEK293T cells, which were then treated with etoposide (10 μM), adriamycin (1 μM), UV irradiation (10 J/m 2 ) and cisplatin (50 or 100 μM) for 24 h. Flag-PFKFB3 was immunoprecipitated with Flag beads and immunoblotting was performed with the antibodies indicated. Relative PFKFB3 K472 acetylation and phosphorylation were normalized by Flag protein. b The amino acid sequence near K472 of PFKFB3 displays high similarity with the sequence near K320 of TP53. c Cisplatin treatment induces PFKFB3 cytoplasmic accumulation. HEK293T cells were treated with or without cisplatin (50 μM) for 24 h before harvest. Cells were then suspended in PBS and treated with a gradient concentration of digitonin. Supernatant and precipitate were collected for immunoblotting with indicated antibodies. S, supernatant; P, precipitate. d Cisplatin induces K472 acetylation and S461 phosphorylation of endogenous PFKFB3. Endogenous PFKFB3 protein was purified from HEK293T cells after cisplatin treatment as indicated for 24 h. e Cisplatin or etoposide treatment enhances K472 acetylation in SIRT1 knockout cells. Endogenous PFKFB3 protein were purified from WT or SIRT1 knockout HEK293T cells treated with EX527 (10 μM), cisplatin (50 μM) or etoposide (10 μM) for 24 h. f Combined knockdown of PCAF and GCN5 abolishes cisplatin- or etoposide-induced PFKFB3 K472 acetylation. HEK293T cells were transfected with siRNAs targeting PCAF and GCN5. After 60 h, cells were treated with cisplatin (50 μM) or etoposide (10 μM) for 24 h. g , h Cisplatin treatment induces pan-acetylation of PCAF and GCN5. Flag-tagged PCAF or GCN5 was expressed in HEK293T cells, which were treated with cisplatin for the duration indicated at a concentration of 50 μM. Relative pan-acetylation level of PCAF or GCN5 was normalized by Flag protein. i Cisplatin increases acetyltransferase activity of PCAF and GCN5. Flag-tag PCAF or GCN5 was purified from HEK293T cells treated with or without cisplatin (50 μM) for 24 h, then incubated with recombinant His-PFKFB3 in acetylation assay buffer. Purified proteins visualized by Coomassie blue staining are shown (lower panel). Data are representative of at least two independent experiments

Journal: Nature Communications

Article Title: Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis

doi: 10.1038/s41467-018-02950-5

Figure Lengend Snippet: Cisplatin induces K472 acetylation and S461 phosphorylation of PFKFB3. a DNA damage signals induced PFKFB3 K472 acetylation. Flag-tagged PFKFB3 was expressed in HEK293T cells, which were then treated with etoposide (10 μM), adriamycin (1 μM), UV irradiation (10 J/m 2 ) and cisplatin (50 or 100 μM) for 24 h. Flag-PFKFB3 was immunoprecipitated with Flag beads and immunoblotting was performed with the antibodies indicated. Relative PFKFB3 K472 acetylation and phosphorylation were normalized by Flag protein. b The amino acid sequence near K472 of PFKFB3 displays high similarity with the sequence near K320 of TP53. c Cisplatin treatment induces PFKFB3 cytoplasmic accumulation. HEK293T cells were treated with or without cisplatin (50 μM) for 24 h before harvest. Cells were then suspended in PBS and treated with a gradient concentration of digitonin. Supernatant and precipitate were collected for immunoblotting with indicated antibodies. S, supernatant; P, precipitate. d Cisplatin induces K472 acetylation and S461 phosphorylation of endogenous PFKFB3. Endogenous PFKFB3 protein was purified from HEK293T cells after cisplatin treatment as indicated for 24 h. e Cisplatin or etoposide treatment enhances K472 acetylation in SIRT1 knockout cells. Endogenous PFKFB3 protein were purified from WT or SIRT1 knockout HEK293T cells treated with EX527 (10 μM), cisplatin (50 μM) or etoposide (10 μM) for 24 h. f Combined knockdown of PCAF and GCN5 abolishes cisplatin- or etoposide-induced PFKFB3 K472 acetylation. HEK293T cells were transfected with siRNAs targeting PCAF and GCN5. After 60 h, cells were treated with cisplatin (50 μM) or etoposide (10 μM) for 24 h. g , h Cisplatin treatment induces pan-acetylation of PCAF and GCN5. Flag-tagged PCAF or GCN5 was expressed in HEK293T cells, which were treated with cisplatin for the duration indicated at a concentration of 50 μM. Relative pan-acetylation level of PCAF or GCN5 was normalized by Flag protein. i Cisplatin increases acetyltransferase activity of PCAF and GCN5. Flag-tag PCAF or GCN5 was purified from HEK293T cells treated with or without cisplatin (50 μM) for 24 h, then incubated with recombinant His-PFKFB3 in acetylation assay buffer. Purified proteins visualized by Coomassie blue staining are shown (lower panel). Data are representative of at least two independent experiments

Article Snippet: Antibody against acetyl-p53 (Lys320, 06–1283, 1:1000) was purchased from Merck Millipore.

Techniques: Phospho-proteomics, Irradiation, Immunoprecipitation, Western Blot, Sequencing, Concentration Assay, Purification, Knock-Out, Knockdown, Transfection, Activity Assay, FLAG-tag, Incubation, Recombinant, Acetylation Assay, Staining